What you will find in this eBook

Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery. The integration of three-dimensional (3D) assay models is becoming more widespread to drive translational biology. Specifically, 3D cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics.

This ebook highlights the 3D cell culture imaging and analysis advantage and includes a few 3D cell model applications as well as tips and techniques for optimizing your 3D assay workflow.

Applications

• Physiologically-relevant tissue models using high-throughput organ-on-a-chip platform
• Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
• High-content assay for morphological characterization of neuronal development in 3D matrix
• Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
• Using spheroid models to develop relevant highthroughput assays with quantitative endpoints

Tips and Techniques

• Automated 3D cell culture and screening by imaging and flow cytometry
• Automation of 3D spheroid cultures in ultra-low attachment plates
• Multi-parameter imaging assay for measuring toxicity in a tumor model
• Measuring mitochondrial shape and distribution
• Increase sensitivity in no-wash assays using confocal imaging

Sample pages

SIMPLIFY 3D CELL CULTURE IMAGING AND ANALYSIS

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