Enhanced development of virus-specific hybridomas
Double-stranded DNA (ds-DNA) viruses cause a variety of disease in humans. Species within the order of Herpesvirus and the families Adenovirus and Papillomavirus can cause symptoms in humans with similar presentations. Furthermore, serological and protein-based diagnostic confirmation for a particular virus may be complicated due to antigen conservation within viral genomes. A high degree of proteomic homology and serum cross reactivities lead to poor differentiation between individual species of ds-DNA viruses. The purpose of the research was to identify an optimal producer cell line that secrets a highly specific, non-cross-reactive monoclonal antibody to be used in biotherapeutics development.