HTRF assay

HTRF assays from Cisbio have evolved to encompass the areas of G protein-coupled receptors, kinases and cell signaling, epigenetics, and biomarkers. A wide variety of toolbox reagents and kits are available to address protein-protein interactions, nuclear receptor assays, receptor dimerization, ligand binding, receptor internalization, enzyme assays, and many more.

HTRF involves the use of two fluorophores, a donor and an acceptor, to label proteins or other biomolecules of interest. When the biomolecules interact, their proximity enables fluorescence resonance energy transfer (FRET) to occur from the donor to the acceptor fluorophore. Using long-lifetime fluorescent lanthanide cryptates as donors allows time-resolved detection of HTRF assays, minimizing short-lived background fluorescence from compounds and other materials. The detection of time-resolved FRET (TR-FRET) with a microplate reader provides a readout of biomolecular binding.