IP-One assays, Gαq/11-coupled receptors
Phospholipase C (PLC) is another common target of activated G proteins and is critical to a variety of processes. For example, thrombin receptors in platelets use this pathway to promote blood clotting. Cisbio IP-One competitive immunoassays detect the accumulation of inositol monophosphate (IP1), a stable downstream metabolite of IP3 induced by the stimulation of PLC following Gαq/11-coupled receptor activation. This kit allows direct characterization of receptor agonists and antagonists, in both adherent and suspension cells.