Dated: Jun 02, 2023
Publication Name: Biosensors and Biomolecular Electronics
Citations : Universal CRISPR/Cas12a-associated aptasensor suitable for rapid detection of small proteins with a plate reader
With the discovery of the collateral cleavage activity, CRISPR/Cas12a has recently been identified as a key enabling approach in novel DNA biosensor development. Despite its remarkable success in nucleic acid detection, realizing a universal CRISPR/Cas biosensing system for non-nucleic acid targets remains challenging, particularly at extremely high sensitivity ranges for analyte concentrations lower than the pM level. DNA aptamers can be designed to bind to a range of specific target molecules, such as proteins, small molecules, and cells, with high affinity and specificity through configuration changes.
Contributors: Yi Li, Linyang Liu, Laicong Qiao and Fei Deng